Abstract
A soil metagenomic library carried in pJC8 (an IncP cosmid) was used for functional complementation for β-galactosidase activity in both α-Proteobacteria (Sinorhizobium meliloti) and γ-Proteobacteria (Escherichia coli). One β-galactosidase, encoded by overlapping clones selected in both hosts, was identified as a member of glycoside hydrolase family 2. ORFs obviously encoding possible β-galactosidases were not identified in 19 other clones that were only able to complement S. meliloti. Based on low sequence similarity to known glycoside hydrolases but not β-galactosidases, three ORFs were examined further. Biochemical analysis confirmed that all encoded β-galactosidase activity. Bioinformatic and structural modeling implied that Lac161_ORF10 protein represented a novel enzyme family with a five-bladed propeller glycoside hydrolase domain.
Abbreviations
- CmR
- (chloramphenicol resistant)
- GmR
- (gentamicin resistant)
- KmR
- (kanamycin resistant)
- NmR
- (neomycin resistant)
- RifR
- (rifampicin resistant)
- SmR
- (streptomycin resistant)
- TcR
- (tetracycline resistant)