Abstract
The marine microalga Ostreococcus is considered to depend on the methionine synthase METH and its methylated cobalamin cofactor for methionine synthesis. Here I describe minimal media lacking both cobalt and cobalamin yet suitable for clonal growth of Ostreococcus tauri. Because Ostreococcus lacks the methylcobalamin-independent methionine synthase METE, Ostreococcus growth without cobalamin is unexplained.
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