Abstract
- EDTA
- ethylenediaminetetraacetic acid
- FBS
- fetal bovine serum
- MFI
- mean fluorescence intensity
- moDC
- monocyte-derived dendritic cells
- PBMC
- peripheral blood mononuclear cells
Autologous serum or plasma are often used as a supplementation in culture media for autoimmune disease studies. They contain many biologically and immunologically active molecules that might be involved in the development of these diseases. We examined the effect of different cultivating media supplemented with autologous serum and two types of plasma obtained from heparin and EDTA coated tubes on monocyte-derived dendritic cells viability. Our results show that medium with plasma from EDTA coated tubes is unsuitable for cultivation of myeloid cells due to the absence of bivalent ions and that the type of sampling tubes used for blood collection might be critical for successful experiment.
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