Abstract
Interleukin 18 (IL18) is known to induce the expression of interferon-γ (IFNG), but its effects on T cell proliferation and costimulation are not completely understood. In this study, we demonstrate that ectopic expression of IL18 in CART cells caused significant T cell proliferation in vitro and in vivo, and enhanced antitumor effects in xenograft models. Moreover, IL18 mediated T cell expansion required neither tumor antigen nor CAR expression, and produced severe GVHD in NSG mice. Furthermore, recombinant IL18 costimulated IFNG secretion and proliferation of anti-CD3 beads treated T cells. Interestingly, IL18 costimulation could expand purified CD4 T cells, but not CD8 T cells. However, CD8 T cells proliferated greater than CD4 T cells in magnitude within bulk T cells, suggesting CD4 help effect was involved. Using CRISPR/Cas9 gene editing, we confirmed that IL18-driven expansion was both TCR and IL18 receptor (IL18R) dependent. Importantly, we demonstrated that TCR-deficient, IL18-expressing CD19 CART cells exhibited remarkable proliferation and persistent antitumor activity against CD19-expressing tumor cells in vivo. Finally, we describe APACHE T cells, a novel strategy for coupling IL18 expression in CART cells to antigen stimulation, thereby limiting potential toxicity associated with persistent IL18 production. In sum, our study supports human IL18 as a T cell costimulatory cytokine for fueling CART therapy.
Abbreviation
- TCR
- T Cell Receptor
- CAR
- Chimeric Antigen Receptor
- IL18-CART
- IL18 producing CART
- CD19-IL18 CART
- IL18 producing CD19 CART
- SS1-IL18 CART
- IL18 producing SS1 CART
- TIL
- Tumor Infiltrating Lymphocytes
- GVHD
- Graft-Versus-Host Disease
- CRISPR
- Clustered regularly interspaced short palindromic repeats
- Cas9
- CRISPR associated protein 9
- IL18R
- Knockout
- KO
- IL18 Receptor
- NFAT
- Nuclear Factor of Activated T-cells
- aAPC
- artificial Antigen Presenting Cells