Abstract
Loss of function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, random sequence labels (RSLs) were incorporated into the guide-library. RSLs function as internal replicates for robust and reproducible hit calling, and act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing (MPLT) and true dropout screening.
Copyright
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