Abstract
We present ethoscopes, machines for high-throughput ethomics in Drosophila and other small animals. Ethoscopes provide four unique features: they provide a software and hardware solution that is reproducible, scalable and open-source; they perform not just real-time tracking, but faithful real-time profiling of behaviour using a supervised machine learning algorithm; they can stimulate flies in a feedback-loop mode; they are highly customisable and open source.
Authors’ contributions QG, LGR and GFG designed the platform; QG and LGR wrote the software; QG and EJB performed the experiments; ARJ contributed the optomotor module; ASF contributed the AGO module; all authors contributed the manuscript.
Competing interests The authors declare that no competing interests exist.