Abstract
RNA sequencing is increasingly performed with less starting material and at a higher sample throughput, e.g. to analyse single-cell transcriptomes. In this context, unique molecular identifiers (UMIs) are used to reduce amplification noise and sample-specific barcodes are used to track libraries. Here, we present a fast and flexible pipeline to process data from such RNA-seq protocols.
Availability: https://github.com/sdparekh/zUMIs
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.