Abstract
We present here an improved protocol for expansion microscopy, which increases the expansion factor from ~4x to ~10x. This protocol, which we termed X10 microscopy, achieves multi-colour imaging at a resolution of at least 30 nm, using off-the-shelf reagents and conventional epifluorescence microscopes. This enables a level of imaging detail rivalling that of much more challenging methods, such as STED, STORM and iExM, in both cell cultures and tissues.
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