PT  - JOURNAL ARTICLE
AU  - Luwen Ning
AU  - Guan Wang
AU  - Zhoufang Li
AU  - Wen Hu
AU  - Qingming Hou
AU  - Yin Tong
AU  - Meng Zhang
AU  - Li Qin
AU  - Xiaoping Chen
AU  - Hengye Man
AU  - Pinghua Liu
AU  - Jiankui He
TI  - Quantitative comparison of single-cell sequencing methods using hippocampal neurons
AID  - 10.1101/004366
DP  - 2014 Jan 01
TA  - bioRxiv
PG  - 004366
4099  - http://biorxiv.org/content/early/2014/04/21/004366.short
4100  - http://biorxiv.org/content/early/2014/04/21/004366.full
AB  - Single-cell genomic analysis has grown rapidly in recent years and will find widespread applications in various fields of biology, including cancer biology, development, immunology, pre-implantation genetic diagnosis, and neurobiology. In this study, we amplified genomic DNA from individual hippocampal neurons using one of three single-cell DNA amplification methods (multiple annealing and looping-based amplification cycles (MALBAC), multiple displacement amplification (MDA), and GenomePlex whole genome amplification (WGA4)). We then systematically evaluated the genome coverage, GC-bias, reproducibility, and copy number variations among individual neurons. Our results showed that single-cell genome sequencing results obtained from the MALBAC and WGA4 methods are highly reproducible and have a high success rate. Chromosome-level and subchromosomal-level copy number variations among individual neurons can be detected.