@article {Haghverdi041384, author = {Laleh Haghverdi and Maren B{\"u}ttner and F. Alexander Wolf and Florian Buettner and Fabian J. Theis}, title = {Diffusion pseudonyme robustly reconstructs lineage branching}, elocation-id = {041384}, year = {2016}, doi = {10.1101/041384}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Single-cell gene expression profiles of differentiating cells encode their intrinsic latent temporal order. We describe an efficient way to robustly estimate this order according to a diffusion pseudotime, which measures transitions on all length scales between cells using diffusion-like random walks. This allows us to identify cells that undergo branching decisions or are in metastable states, and thereby genes differentially regulated at these states.}, URL = {https://www.biorxiv.org/content/early/2016/02/29/041384}, eprint = {https://www.biorxiv.org/content/early/2016/02/29/041384.full.pdf}, journal = {bioRxiv} }