PT  - JOURNAL ARTICLE
AU  - Hamid Bahrami
AU  - Saeed Daneshmandi
AU  - Hasan Heidarnazhad
AU  - Ali Akbar Pourfathollah
TI  - Lack of association between Toll Like Receptor-2 & Toll Like Receptor-4 Gene Polymorphisms and Iranian Asthmatics risk or features
AID  - 10.1101/004382
DP  - 2014 Jan 01
TA  - bioRxiv
PG  - 004382
4099  - http://biorxiv.org/content/early/2014/04/28/004382.short
4100  - http://biorxiv.org/content/early/2014/04/28/004382.full
AB  - Background Asthma as chronic inflammatory airway disease is considered to be the most common chronic disease that is involving genetic and environmental factors. Toll like receptors (TLRs) and other inflammatory mediators are important in modulation of inflammation. In this study we evaluated the role of TLR2 Arg753Gln and TLR4 Asp299Gly polymorphisms in the asthma susceptibility, progress, control levels and lung functions in Iranian subjects.Methods On 99 asthmatic patients and 120 normal subjects, TLR2 Arg753Gln and TLR4 Asp299Gly polymorphism were evaluated by PCR-RFLP method recruiting Msp1 and Nco1 restriction enzymes, respectively. IgE serum levels by ELISA technique were determined and asthma diagnosis, treatment and control levels were considered using standard schemes and criteria.Results Our results indicated that the genotype and allele frequencies of the TLR2 Arg753Gln and TLR4 Asp299Gly polymorphisms were not significantly different between control subjects and asthmatics (p > 0.05) or even in asthma features such as IgE levels, asthma history and pulmonary factors (p > 0.05).Conclusions Meanwhile some previous studies indicated TLRs and their polymorphisms role in asthma incidence and features, our data demonstrated that TLR2 Arg753Gln and TLR4 Asp299Gly gene variants were not risk factor of asthma or its features in Iranian patients. Genetic complexity, ethnicity, influence of other genes or polymorphisms may overcome these polymorphisms in our asthmatics.