TY - JOUR T1 - On the Reproducibility of TCGA Ovarian Cancer MicroRNA Profiles JF - bioRxiv DO - 10.1101/000315 SP - 000315 AU - Ying-Wooi Wan AU - Claire M. Mach AU - Genevera I. Allen AU - Matthew L. Anderson AU - Zhandong Liu Y1 - 2013/01/01 UR - http://biorxiv.org/content/early/2013/11/13/000315.abstract N2 - Dysregulated microRNA (miRNA) expression is a well-established feature of human cancer. However, the role of specific miRNAs in determining cancer outcomes remains unclear. Using Level 3 expression data from the Cancer Genome Atlas (TCGA), we identified 61 miRNAs that are associated with overall survival in 469 ovarian cancers profiled by microarray (p<0.01). We also identified 12 miRNAs that are associated with survival when miRNAs were profiled in the same specimens using Next Generation Sequencing (miRNA-Seq) (p<0.01). Surprisingly, only 1 miRNA transcript is associated with ovarian cancer survival in both datasets. Our analyses indicate that this discrepancy is due to the fact that miRNA levels reported by the two platforms correlate poorly, even after correcting for potential issues inherent to signal detection algorithms. Further investigation is warranted. ER -