TY - JOUR T1 - Natural history of Asian lineage Zika virus infection in macaques JF - bioRxiv DO - 10.1101/046334 SP - 046334 AU - Dawn M. Dudley AU - Matthew T. Aliota AU - Emma L. Mohr AU - Andrea M. Weiler AU - Gabrielle Lehrer-Brey AU - Kim L. Weisgrau AU - Mariel S. Mohns AU - Meghan E. Breitbach AU - Mustafa N. Rasheed AU - Christina M. Newman AU - Dane D. Gellerup AU - Louise H. Moncla AU - Jennifer Post AU - Nancy Schultz-Darken AU - Josh A. Eudailey AU - M. Anthony Moody AU - Sallie R. Permar AU - Shelby L. O’Connor AU - Eva G. Rakasz AU - Heather A. Simmons AU - Saverio Capuano III AU - Thaddeus G. Golos AU - Jorge E. Osorio AU - Thomas C. Friedrich AU - David H. O’Connor Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/03/30/046334.abstract N2 - Infection with Asian lineage Zika virus has been associated with Guillain-Barre syndrome and fetal microcephaly1–4. Here we show that rhesus macaques are susceptible to infection by an Asian lineage Zika virus isolate that shares more than 99% nucleotide identity with strains currently circulating in the Americas. Following subcutaneous inoculation, Zika virus RNA was detected in plasma one-day post infection (dpi) in all animals (N = 3). Plasma viral loads peaked above 1 × 106 viral RNA copies/mL in two of three animals. Viral RNA was also present in saliva, urine, and cerebrospinal fluid (CSF), consistent with case reports from infected humans. Zika virus RNA persisted in both plasma and urine at low levels for more than two weeks. Infection was associated with transient increases in proliferating natural killer cells, CD8+ and CD4+ T cells, and plasmablasts, suggesting pathogen sensing by the immune system. These data establish that Asian lineage Zika virus infection in rhesus macaques provides a relevant animal model for human infection. Furthermore, because fetal development is well characterized in rhesus macaques, infections in pregnant macaques will enable important studies of fetal defects associated with Zika virus. ER -