TY - JOUR T1 - ExM-STORM: Expansion Single Molecule Nanoscopy JF - bioRxiv DO - 10.1101/049403 SP - 049403 AU - Zhisong Tong AU - Paolo Beuzer AU - Qing Ye AU - Josh Axelrod AU - Zhenmin Hong AU - Hu Cang Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/04/20/049403.abstract N2 - Recently, Chen et al. invented Expansion Microscopy, or ExM, which imprints a specimen’s features of interest onto a polymer hydrogel network and expands the network homogeneously to magnify sub-diffraction-limit features. However, ExM bleaches massively, 50-100% of the fluorescent molecules; thus it is not suited for single-molecule imaging. Here, we describe improved ExM that is free of bleaching, and thus can be synergically integrated with single-molecule nanoscopy, STORM. ER -