RT Journal Article
SR Electronic
T1 Direct binding to RPA-coated ssDNA allows recruitment of the ATR activator TopBP1 to sites of DNA damage
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 050013
DO 10.1101/050013
A1 Julyana Acevedo
A1 Shan Yan
A1 Matthew Michael
YR 2016
UL http://biorxiv.org/content/early/2016/04/23/050013.abstract
AB A critical event for the ability of cells to tolerate DNA damage and replication stress is activation of the ATR kinase. ATR activation is dependent on the BRCT repeat-containing protein TopBP1. Previous work has shown that recruitment of TopBP1 to sites of DNA damage and stalled replication forks is necessary for downstream events in ATR activation, however the mechanism for this recruitment was not known. Here, we use protein binding assays and functional studies in Xenopus egg extracts to show that TopBP1 makes a direct interaction, via its BRCT2 domain, with RPA-coated ssDNA. We identify a point mutant that abrogates this interaction, and show that this mutant fails to accumulate at sites of DNA damage, and that the mutant cannot activate ATR. These data thus supply a mechanism for how the critical ATR activator, TopBP1, senses DNA damage and stalled replication forks to initiate assembly of checkpoint signaling complexes.