RT Journal Article
SR Electronic
T1 A data acquisition and analysis pipeline for scanning angle interference microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 050468
DO 10.1101/050468
A1 Catherine B. Carbone
A1 Ronald D. Vale
A1 Nico Stuurman
YR 2016
UL http://biorxiv.org/content/early/2016/04/26/050468.abstract
AB We describe open source software and hardware tools for calibrating, acquiring, and analyzing images for scanning angle interference microscopy (SAIM) analysis. These tools make it possible for any user with a TIRF microscope equipped with a motorized illumination unit to generate reconstructed images with nanometer precision in the axial (z) direction and diffraction-limited resolution in the lateral (xy) plane.