RT Journal Article
SR Electronic
T1 Septins are involved at the early stages of macroautophagy in <em>S. cerevisiae</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 043133
DO 10.1101/043133
A1 Gaurav Barve
A1 Shreyas Sridhar
A1 Amol Aher
A1 Sunaina Singh
A1 Lakshmeesha K.N.
A1 Ravi Manjithaya
YR 2016
UL http://biorxiv.org/content/early/2016/05/04/043133.abstract
AB Autophagy is a conserved cellular degradation pathway wherein a double membrane vesicle, called as an autophagosome captures longlived proteins, damaged or superfluous organelles and delivers to the lysosome for degradation1. We have identified a novel role for septins in autophagy. Septins are GTP-binding proteins that localize at the bud-neck and are involved in cytokinesis in budding yeast2. We show that septins under autophagy prevalent conditions are majorly localized to the cytoplasm in the form of punctate structures. Further, we report that septins not only localize to pre-autophagosomal structure (PAS) but also to autophagosomes in the form of punctate structures. Interestingly, septins also form small non-canonical rings around PAS during autophagy. Furthermore, we observed that in one of the septin Ts" mutant, cdc10-5, the anterograde trafficking of Atg9 was affected at the non-permissive temperature (NPT). All these results suggest a role of septins in early stages of autophagy during autophagosome formation.