TY - JOUR T1 - Ayurvedic <em>Amalaki Rasayana</em> promotes improved stress tolerance and thus has anti-aging effects in <em>Drosophila melanogaster</em> JF - bioRxiv DO - 10.1101/050476 SP - 050476 AU - Vibha Dwivedi AU - Subhash C. Lakhotia Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/05/04/050476.abstract N2 - Ethnopharmacological relevance Amalaki Rasayana (AR) is a common Ayurvedic herbal formulation of Phyllanthus emblica fruits and other ingredients and is used for general good health and healthy aging. We earlier reported it to improve life history traits and to suppress neurodegeneration as well as induced apoptosis in Drosophila.Aim of the study To examine effects of dietary AR supplement on cell stress responses in Drosophila melanogaster.Materials and methods Larvae/flies, reared on normal food or on that supplemented with 0.5% (w/v) AR, were exposed to crowding, thermal or oxidative stress and examined for survival, stress tolerance and levels of lipid peroxides, SOD and HSPs.Results Wild type larvae/flies reared on AR supplemented food survived the various cell stresses much better than those reared on normal food. AR-fed mutant park13 or DJ-1βDelta93 (Parkinson’s disease model) larvae, however, showed only partial or no protection, respectively, against paraquat-induced oxidative stress, indicating essentiality of DJ-1β for AR mediated oxidative stress tolerance. AR feeding reduced the accumulation of reactive oxygen species (ROS) and lipid peroxidation even in aged (35 day old) wild type flies while enhancing superoxide dismutase (SOD) activity. We show for the first time that while Hsp70 or Hsp83 expression under normal or stress conditions was not differentially affected by AR feeding, Hsp27 levels were elevated in AR fed wild type control as well as heat shocked larvae. Therefore, besides the known anti-oxidant activity of Phyllanthus emblica fruits, dietary AR also enhances cellular levels of Hsp27.Conclusion In the context of the reported “anti-aging” and “healthy-aging” effects of AR, the present in vivo study on a model organisms shows that AR feeding significantly improves tolerance to a variety of cell stresses through reduced ROS and lipid peroxidation and enhanced SOD activity and Hsp27. Such improved cellular defences following dietary AR provide better homeostasis and thereby improve the life-span and quality of organism’s life. ER -