RT Journal Article SR Electronic T1 Rapidly evolving homing CRISPR barcodes JF bioRxiv FD Cold Spring Harbor Laboratory SP 055863 DO 10.1101/055863 A1 Reza Kalhor A1 Prashant Mali A1 George M. Church YR 2016 UL http://biorxiv.org/content/early/2016/05/27/055863.abstract AB We present here an approach for engineering evolving DNA barcodes in living cells. The methodology entails use of a homing guide RNA (hgRNA) scaffold that directs the Cas9-hgRNA complex to target the DNA locus of the hgRNA itself. We show this homing CRISPR-Cas9 system acts as an expressed evolving genetic barcode, and corresponding small RNAs can be assayed as single molecules in situ. This integrated approach will have wide ranging applications, such as in deep lineage tracing, cellular barcoding, molecular recording, dissecting cancer biology, and connectome mapping.