RT Journal Article
SR Electronic
T1 Massively parallel whole-organism lineage tracing using CRISPR/Cas9 induced genetic scars
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 056499
DO 10.1101/056499
A1 Jan Philipp Junker
A1 Bastiaan Spanjaard
A1 Josi Peterson-Maduro
A1 Anna Alemany
A1 Bo Hu
A1 Maria Florescu
A1 Alexander van Oudenaarden
YR 2016
UL http://biorxiv.org/content/early/2016/06/01/056499.abstract
AB A key goal of developmental biology is to understand how a single cell transforms into a full-grown organism consisting of many cells. Although impressive progress has been made in lineage tracing using imaging approaches, analysis of vertebrate lineage trees has mostly been limited to relatively small subsets of cells. Here we present scartrace, a strategy for massively parallel whole-organism lineage tracing based on Cas9 induced genetic scars in the zebrafish.