TY - JOUR T1 - Comments on: “A comprehensive repertoire of tRNA-derived fragments in prostate cancer” JF - bioRxiv DO - 10.1101/061572 SP - 061572 AU - Rogan Magee AU - Phillipe Loher AU - Aristeidis G. Telonis AU - Yohei Kirino AU - Isidore Rigoutsos Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/07/07/061572.abstract N2 - We evaluated the deep-sequencing (RNA-seq) data from human prostate tissue that were reported in [1] and the tRNA-derived fragments described in the original analysis. Our study of the same RNA-seq datasets reveals a considerably different pool of tRNA fragments, many of them with higher abundances than the fragments reported in [1]. We also evaluated the q-PCR approach proposed in [1]. As the approach lacks 5’-endpoint specificity, it will not estimate correctly the abundance of many of the tRFs that are present in the sampled RNA populations from human prostate tissue. ER -