PT  - JOURNAL ARTICLE
AU  - Sergiy M. Nadtochiy
AU  - Xenia Schafer
AU  - Dragony Fu
AU  - Keith Nehrke
AU  - Joshua Munger
AU  - Paul S. Brookes
TI  - Acidic pH is a Metabolic Switch for 2-Hydroxyglutarate Generation and Signaling
AID  - 10.1101/051599
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 051599
4099  - http://biorxiv.org/content/early/2016/07/22/051599.short
4100  - http://biorxiv.org/content/early/2016/07/22/051599.full
AB  - 2-hydroxyglutarate (2-HG) is an important epigenetic regulator, with potential roles in cancer and stem cell biology. The D (R) enantiomer (D-2-HG) is an oncometabolite generated from αketoglutarate (α-KG) by mutant isocitrate dehydrogenase (ICDH), while L (S) 2-HG is generated by lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in response to hypoxia. Since acidic pH is a common feature of hypoxia, as well as tumor and stem cell microenvironments, we hypothesized that pH may regulate cellular 2-HG levels. Herein we report that cytosolic acidification under normoxia moderately elevated 2-HG in cells, and boosting endogenous substrate α-KG levels further stimulated this elevation. Studies with isolated LDH-1 and MDH-2 revealed that generation of 2-HG by both enzymes was stimulated several-fold at acidic pH, relative to normal physiologic pH. In addition, acidic pH was found to inhibit the activity of the mitochondrial L-2-HG removal enzyme L-2-HG dehydrogenase, and to stimulate the reverse reaction of ICDH (carboxylation of αKG to isocitrate). Furthermore, since acidic pH is known to stabilize hypoxia-inducible factor (HIF), and 2-HG is a known inhibitor of HIF prolyl hydroxylases, we hypothesized that 2-HG may be required for acid-induced HIF stabilization. Accordingly, cells stably over-expressing L-2HGDH exhibited a blunted HIF response to acid. Together these results suggest that acidosis is an important and previously overlooked regulator of 2-HG accumulation and other oncometabolic events, with implications for HIF signaling.