PT  - JOURNAL ARTICLE
AU  - Robert Vaser
AU  - Ivan Sović
AU  - Niranjan Nagarajan
AU  - Mile Šikić
TI  - Fast and accurate <em>de novo</em> genome assembly from long uncorrected reads
AID  - 10.1101/068122
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 068122
4099  - http://biorxiv.org/content/early/2016/08/05/068122.short
4100  - http://biorxiv.org/content/early/2016/08/05/068122.full
AB  - The assembly of long reads from Pacific Biosciences and Oxford Nanopore Technologies typically requires resource intensive error correction and consensus generation steps to obtain high quality assemblies. We show that the error correction step can be omitted and high quality consensus sequences can be generated efficiently with a SIMD accelerated, partial order alignment based stand-alone consensus module called Racon. Based on tests with PacBio and Oxford Nanopore datasets we show that Racon coupled with Miniasm enables consensus genomes with similar or better quality than state-of-the-art methods while being an order of magnitude faster.Racon is available open source under the MIT license at https://github.com/isovic/racon.git.