TY - JOUR T1 - Highly parallel direct RNA sequencing on an array of nanopores JF - bioRxiv DO - 10.1101/068809 SP - 068809 AU - Daniel R. Garalde AU - Elizabeth A. Snell AU - Daniel Jachimowicz AU - Andrew J. Heron AU - Mark Bruce AU - Joseph Lloyd AU - Anthony Warland AU - Nadia Pantic AU - Tigist Admassu AU - Jonah Ciccone AU - Sabrina Serra AU - Jemma Keenan AU - Samuel Martin AU - Luke McNeill AU - Jayne Wallace AU - Lakmal Jayasinghe AU - Chris Wright AU - Javier Blasco AU - Botond Sipos AU - Stephen Young AU - Sissel Juul AU - James Clarke AU - Daniel J Turner Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/08/12/068809.abstract N2 - Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing – information that is useful for understanding the status and function of a sample. Oxford Nanopore Technologies’ sequencing technology is capable of electronically analysing a sample’s DNA directly, and in real-time. In this manuscript we demonstrate the ability of an array of nanopores to sequence RNA directly, and we apply it to a range of biological situations. Nanopore technology is the only available sequencing technology that can sequence RNA directly, rather than depending on reverse transcription and PCR. There are several potential advantages of this approach over other RNA-seq strategies, including the absence of amplification and reverse transcription biases, the ability to detect nucleotide analogues and the ability to generate full-length, strand-specific RNA sequences. Direct RNA sequencing is a completely new way of analysing the sequence of RNA samples and it will improve the ease and speed of RNA analysis, while yielding richer biological information. ER -