TY - JOUR T1 - The Detailed 3D Multi-Loop Aggregate/Rosette Chromatin Architecture and Functional Dynamic Organization of the Human and Mouse Genomes JF - bioRxiv DO - 10.1101/064642 SP - 064642 AU - Tobias A. Knoch AU - Malte Wachsmuth AU - Nick Kepper AU - Michael Lesnussa AU - Anis Abuseiris AU - A. M. Ali Imam AU - Petros Kolovos AU - Jessica Zuin AU - Christel E. M. Kockx AU - Rutger W. W. Brouwer AU - Harmen J. G. van de Werken AU - Wilfred F. J. van IJken AU - Kerstin S. Wendt AU - Frank G. Grosveld Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/08/15/064642.abstract N2 - The dynamic three-dimensional chromatin architecture of genomes and its co-evolutionary connection to its function – the storage, expression, and replication of genetic information – is still one of the central issues in biology. Here, we describe the much debated 3D-architecture of the human and mouse genomes from the nucleosomal to the megabase pair level by a novel approach combining selective high-throughput high-resolution chromosomal interaction capture (T2C), polymer simulations, and scaling analysis of the 3D-architecture and the DNA sequence: The genome is compacted into a chromatin quasi-fibre with ∼5±1 nucleosomes/11nm, folded into stable ∼30-100 kbp loops forming stable loop aggregates/rosettes connected by similar sized linkers. Minor but significant variations in the architecture are seen between cell types/functional states. The architecture and the DNA sequence show very similar fine-structured multi-scaling behaviour confirming their co-evolution and the above. This architecture, its dynamics, and accessibility balance stability and flexibility ensuring genome integrity and variation enabling gene expression/regulation by self-organization of (in)active units already in proximity. Our results agree with the heuristics of the field and allow “architectural sequencing” at a genome mechanics level to understand the inseparable systems genomic properties. ER -