PT  - JOURNAL ARTICLE
AU  - María Belén Jerez
AU  - Maximiliano Juri Ayub
TI  - Degradation of non-methylated DNA by MsPJI impairs its usefulness as epigenetic tool
AID  - 10.1101/072090
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 072090
4099  - http://biorxiv.org/content/early/2016/08/29/072090.short
4100  - http://biorxiv.org/content/early/2016/08/29/072090.full
AB  - DNA treatment with sensitive and/or dependent restriction enzymes, followed by PCR amplification is a widely used approach for testing CpG methylation. Recently, MspJI has been characterized as a promisory tool for epigenetic analyses. In the present report, we describe that MspJI shows significant activity against non-methylated DNA, suggesting that additional caution and improvements would be required before applying this enzyme as a routine epigenetic tool.