PT  - JOURNAL ARTICLE
AU  - Matthias Meurer
AU  - Veronika Chevyreva
AU  - Bram Cerulus
AU  - Michael Knop
TI  - The regulatable <em>MAL32</em> promoter in <em>S. cerevisiae</em>: characteristics and tools to facilitate its use
AID  - 10.1101/061127
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 061127
4099  - http://biorxiv.org/content/early/2016/09/02/061127.short
4100  - http://biorxiv.org/content/early/2016/09/02/061127.full
AB  - Here we describe a set of tools to facilitate the use of maltose and the MAL32 promoter for regulated gene expression in yeast, alone or in combination with the GAL1 promoter. Using fluorescent protein reporters we find that under non-inducing conditions the MAL32 promoter exhibits a low basal level of expression, similar to the GAL1 promoter, and that both promoters can be induced independently of each other using the respective sugars, maltose and galactose. While their repression upon glucose addition is immediate and complete, we found that the MAL32 and GAL1 promoter each exhibit distinct induction kinetics. A set of plasmids is available to facilitate the application of the MAL32 promoter for chromosomal modifications using PCR targeting and for plasmid based gene expression.