RT Journal Article
SR Electronic
T1 The regulatable MAL32 promoter in S. cerevisiae: characteristics and tools to facilitate its use
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 061127
DO 10.1101/061127
A1 Matthias Meurer
A1 Veronika Chevyreva
A1 Bram Cerulus
A1 Michael Knop
YR 2016
UL http://biorxiv.org/content/early/2016/09/02/061127.abstract
AB Here we describe a set of tools to facilitate the use of maltose and the MAL32 promoter for regulated gene expression in yeast, alone or in combination with the GAL1 promoter. Using fluorescent protein reporters we find that under non-inducing conditions the MAL32 promoter exhibits a low basal level of expression, similar to the GAL1 promoter, and that both promoters can be induced independently of each other using the respective sugars, maltose and galactose. While their repression upon glucose addition is immediate and complete, we found that the MAL32 and GAL1 promoter each exhibit distinct induction kinetics. A set of plasmids is available to facilitate the application of the MAL32 promoter for chromosomal modifications using PCR targeting and for plasmid based gene expression.