@article {Edgar074252, author = {Robert C. Edgar}, title = {UCHIME2: improved chimera prediction for amplicon sequencing}, elocation-id = {074252}, year = {2016}, doi = {10.1101/074252}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Amplicon sequencing generates chimeric reads which can cause spurious inferences of biological variation. I describe UCHIME2, an update of the popular UCHIME chimera detection algorithm with new modes optimized for high-resolution biological sequence reconstruction ({\textquotedblleft}denoising{\textquotedblright}) and other applications. I show that chimera frequency correlates inversely with divergence, that error-free chimera prediction from sequence is impossible in principle, and that UCHIME2 achieves higher detection accuracy than previous methods.}, URL = {https://www.biorxiv.org/content/early/2016/09/09/074252}, eprint = {https://www.biorxiv.org/content/early/2016/09/09/074252.full.pdf}, journal = {bioRxiv} }