PT - JOURNAL ARTICLE AU - Krzysztof Kiryluk AU - Yifu Li AU - Zina Moldoveanu AU - Hitoshi Suzuki AU - Colin Reily AU - Ping Hou AU - Jingyuan Xie AU - Nikol Mladkova AU - Sindhuri Prakash AU - Clara Fischman AU - Samantha Shapiro AU - Robert A. LeDesma AU - Drew Bradbury AU - Iuliana Ionita-Laza AU - Frank Eitner AU - Thomas Rauen AU - Nicolas Maillard AU - Francois Berthoux AU - Jürgen Floege AU - Nan Chen AU - Hong Zhang AU - Francesco Scolari AU - Robert J. Wyatt AU - Bruce A. Julian AU - Ali G. Gharavi AU - Jan Novak TI - GWAS for serum galactose-deficient IgA1 implicates critical genes of the <em>O</em>-glycosylation pathway AID - 10.1101/076414 DP - 2016 Jan 01 TA - bioRxiv PG - 076414 4099 - http://biorxiv.org/content/early/2016/09/21/076414.short 4100 - http://biorxiv.org/content/early/2016/09/21/076414.full AB - Aberrant O-glycosylation of serum immunoglobulin A1 (IgA1) represents a heritable pathogenic defect in IgA nephropathy, the most common form of glomerulonephritis worldwide, but specific genetic factors involved in its determination are not known. We performed a quantitative GWAS for serum levels of galactose-deficient IgA1 (Gd-IgA1) in 2,633 subjects of European and East Asian ancestry and discovered two genome-wide significant loci, in C1GALT1 (rs13226913, P = 3.2 × 10−11) and C1GALT1C1 (rs5910940, P = 2.7 × 10−8). These genes encode molecular partners essential for enzymatic O-glycosylation of IgA1. We demonstrated that these two loci explain approximately 7% of variability in circulating Gd-IgA1 in Europeans, but only 2% in East Asians. Notably, the Gd-IgA1-increasing allele of rs13226913 is common in Europeans, but rare in East Asians. Moreover, rs13226913 represents a strong cis-eQTL for C1GALT1, which encodes the key enzyme responsible for the transfer of galactose to O-linked glycans on IgA1. By in vitro siRNA knock-down studies, we confirmed that mRNA levels of both C1GALT1 and C1GALT1C1 determine the rate of secretion of Gd-IgA1 in IgA1-producing cells. Our findings provide novel insights into the genetic regulation of O-glycosylation and are relevant not only to IgA nephropathy, but also to other complex traits associated with O-glycosylation defects, including inflammatory bowel disease, hematologic disease, and cancer.Author Summary O-glycosylation is a common type of post-translational modification of proteins; specific abnormalities in the mechanism of O-glycosylation have been implicated in cancer, inflammatory and blood diseases. However, the molecular basis of abnormal O-glycosylation in these complex disorders is not known. We studied the genetic basis of defective O-glycosylation of serum Immunoglobulin A1 (IgA1), which represents the key pathogenic defect in IgA nephropathy, the most common form of primary glomerulonephritis worldwide. We report our results of the first genome-wide association study for this trait using serum assays in 2,633 individuals of European and East Asian ancestry. In our genome scan, we observed two significant signals with large effects, on chromosomes 7p21.3 and Xq24, jointly explaining about 7% of trait variability. These signals implicate two genes that encode molecular partners essential for enzymatic O-glycosylation of IgA1 and mucins, and represent potential new targets for therapy.