RT Journal Article
SR Electronic
T1 Single Cell Phenotyping Reveals Heterogeneity among Haematopoietic Stem Cells Following Infection
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 080416
DO 10.1101/080416
A1 Adam L MacLean
A1 Maia A Smith
A1 Juliane Liepe
A1 Aaron Sim
A1 Reema Khorshed
A1 Nico Scherf
A1 Axel Krinner
A1 Ingo Roeder
A1 Cristina Lo Celso
A1 Michael PH Stumpf
YR 2016
UL http://biorxiv.org/content/early/2016/10/12/080416.abstract
AB The haematopoietic stem cell (HSC) niche provides essential micro-environmental cues for the production and maintenance of HSCs within the bone marrow. During inflammation, haematopoietic dynamics are perturbed, but it is not known whether changes to the HSC-niche interaction occur as a result. We visualise HSCs directly in vivo, enabling detailed analysis of the 3D niche dynamics and migration patterns in murine bone marrow following Trichinella spiralis infection. Spatial statistical analysis of these HSC trajectories reveals two distinct modes of HSC behaviour: (i) a preference for revisiting previously explored space, and (ii) a preference for exploring new space. Whereas HSCs from control donors predominantly follow pattern (i), those from infected mice follow both. Using detailed computational analyses of cell migration tracks and life-history theory, we show that the increased motility of HSCs following infection can, perhaps counterintuitively, enable mice to cope better in deteriorating HSC-niche micro-environments following infection.