PT  - JOURNAL ARTICLE
AU  - Robert C. Edgar
TI  - UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing
AID  - 10.1101/081257
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 081257
4099  - http://biorxiv.org/content/early/2016/10/15/081257.short
4100  - http://biorxiv.org/content/early/2016/10/15/081257.full
AB  - Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for investigating microbial populations. In such experiments, sequence errors caused by PCR and sequencing are difficult to distinguish from true biological variation. I describe UNOISE2, an updated version of the UNOISE algorithm for denoising (error-correcting) Illumina amplicon reads and show that it has comparable or better accuracy than DADA2.