RT Journal Article SR Electronic T1 Experimental and Computational Investigation of the Structure of Peptide Monolayers on Gold Nanoparticles JF bioRxiv FD Cold Spring Harbor Laboratory SP 083204 DO 10.1101/083204 A1 Elena Colangelo A1 Qiubo Chen A1 Adam M. Davidson A1 David Paramelle A1 Michael B. Sullivan A1 Martin Volk A1 Raphaël Lévy YR 2016 UL http://biorxiv.org/content/early/2016/10/25/083204.abstract AB The self-assembly and self-organization of small molecules at the surface of nanoparticles constitute a potential route towards the preparation of advanced protein-like nanosystems. However, their structural characterization, critical to the design of bio-nanomaterials with well-defined biophysical and biochemical properties, remains highly challenging. Here, a computational model for peptide-capped gold nanoparticles is developed using experimentally characterized CALNN-and CFGAILSS-capped gold nanoparticles as a benchmark. The structure of CALNN and CFGAILSS monolayers is investigated by both structural biology techniques and molecular dynamics simulations. The calculations reproduce the experimentally observed dependence of the monolayer secondary structure on peptide capping density and on nanoparticle size, thus giving us confidence in the model. Furthermore, the computational results reveal a number of new features of peptide-capped monolayers, including the importance of sulfur movement for the formation of secondary structure motifs, the presence of water close to the gold surface even in tightly packed peptide monolayers, and the existence of extended 2D parallel β-sheet domains in CFGAILSS monolayers. The model developed here provides a predictive tool that may assist in the design of further bio-nanomaterials.