RT Journal Article SR Electronic T1 Deep Sequencing revealed ‘Plant Like Transcripts’ in mosquito Anopheles culicifacies: an Evolutionary Puzzle JF bioRxiv FD Cold Spring Harbor Laboratory SP 010009 DO 10.1101/010009 A1 Punita Sharma A1 Swati Sharma A1 Ashwani Kumar Mishra A1 Tina Thomas A1 Tanwee Das De A1 Sonia Verma A1 Vandana Kumari A1 Suman Lata Rohilla A1 Namita Singh A1 Kailash C Pandey A1 Rajnikant Dixit YR 2014 UL http://biorxiv.org/content/early/2014/10/03/010009.abstract AB As adult female mosquito’s salivary gland facilitate blood meal uptake and pathogen transmission e.g. Plasmodium, virus etc., a plethora of research has been focused to understand the mosquito-vertebrate-pathogen interactions. Despite the fact that mosquito spends longer time over nectar sugar source, the fundamental question ‘how adult female salivary gland’ manages molecular and functional relationship during sugar vs. blood meal uptake remains unanswered. Currently, we are trying to understand these molecular relationships under dual feeding conditions in the salivary glands of the mosquito Anopheles culicifacies. During functional annotation of salivary transcriptome database, unexpectedly we discovered a cluster of salivary transcripts encoding plant like proteins. Our multiple experimental validations confirmed that Plant like transcripts (PLTs) are of mosquito origin and may encode functional proteins. A comprehensive molecular analysis of the PLTs and ongoing metagenomic analysis of salivary microbiome provide first evidence that how mosquito may have been benefited from its association with plant host and microbes. Future understanding of the underlying mechanism of the feeding associated molecular responses may provide new opportunity to control vector borne diseases.