PT  - JOURNAL ARTICLE
AU  - Makoto Ohira
AU  - David G. Hendrickson
AU  - R. Scott McIsaac
AU  - Nicholas Rhind
TI  - An Estradiol-Inducible Promoter Enables Fast, Graduated Control of Gene Expression in Fission Yeast
AID  - 10.1101/092338
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 092338
4099  - http://biorxiv.org/content/early/2016/12/07/092338.short
4100  - http://biorxiv.org/content/early/2016/12/07/092338.full
AB  - The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β-estradiol-regulated function of the human estrogen receptor, for use in S. pombe. We show that this promoter system, termed Z3EV, turns on quickly, reaches a maximal induction of over 20 fold, and exhibits a linear dose response over its entire induction range. We demonstrate the utility of this system by regulating the mitotic inhibitor Wee1 to create a strain in which cell size is regulated by β-estradiol concentration. This promoter system will be of great utility for experimentally regulating gene expression in fission yeast.