PT - JOURNAL ARTICLE AU - Mark Paul PaĊĦek TI - Cultured <em>Ostreococcus</em> is cobalamin-independent AID - 10.1101/065771 DP - 2016 Jan 01 TA - bioRxiv PG - 065771 4099 - http://biorxiv.org/content/early/2016/12/21/065771.short 4100 - http://biorxiv.org/content/early/2016/12/21/065771.full AB - The marine microalga Ostreococcus is considered to depend on the methionine synthase METH and its methylated cobalamin cofactor for methionine synthesis because it lacks METE, the methylcobalamin-independent methionine synthase. Here I describe minimal media lacking both biotin and cobalamin yet suitable for growth of cultured Ostreococcus.