TY - JOUR T1 - A SUMO-Ubiquitin Relay Recruits Proteasomes to Chromosome Axes to Regulate Meiotic Recombination JF - bioRxiv DO - 10.1101/095711 SP - 095711 AU - H.B.D. Prasada Rao AU - Huanyu Qiao AU - Shubhang K. Bhatt AU - Logan R.J. Bailey AU - Hung D. Tran AU - Sarah L. Bourne AU - Wendy Qiu AU - Anusha Deshpande AU - Ajay N. Sharma AU - Connor J. Beebout AU - Roberto J. Pezza AU - Neil Hunter Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/12/21/095711.abstract N2 - Meiosis produces haploid gametes through a succession of chromosomal events including pairing, synapsis and recombination. Mechanisms that orchestrate these events remain poorly understood. We found that the SUMO-modification and ubiquitin-proteasomes systems regulate the major events of meiotic prophase in mouse. Interdependent localization of SUMO, ubiquitin and proteasomes along chromosome axes was mediated largely by RNF212 and HEI10, two E3 ligases that are also essential for crossover recombination. RNF212-dependent SUMO conjugation effected a checkpoint-like process that stalls recombination by rendering the turnover of a subset of recombination factors dependent on HEI10-mediated ubiquitylation. We propose that SUMO conjugation establishes a precondition for designating crossover sites via selective protein stabilization. Thus, meiotic chromosome axes are hubs for regulated proteolysis via SUMO-dependent control of the ubiquitin-proteasome system.One Sentence Summary Chromosomal events of meiotic prophase in mouse are regulated by proteasome-dependent protein degradation. ER -