RT Journal Article
SR Electronic
T1 Quantitative mRNA Imaging Throughout the Entire <em>Drosophila</em> Brain
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 096388
DO 10.1101/096388
A1 Xi Long
A1 Jennifer Colonell
A1 Allan M Wong
A1 Robert H Singer
A1 Timothée Lionnet
YR 2016
UL http://biorxiv.org/content/early/2016/12/22/096388.abstract
AB We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid, multiplexable and does not require molecular amplification; it allows facile mRNA expression quantification with subcellular resolution on a standard confocal microscope. Using a custom Bessel Beam-Structured Illumination microscope (BB-SIM), we further demonstrate single-mRNA detection across the entire brain sample.