PT  - JOURNAL ARTICLE
AU  - David L. Stern
AU  - Justin Crocker
AU  - Yun Ding
AU  - Nicolas Frankel
AU  - Gretchen Kappes
AU  - Elizabeth Kim
AU  - Ryan Kuzmickas
AU  - Andrew Lemire
AU  - Serge Picard
TI  - Genetic and transgenic reagents for <em>Drosophila simulans, D. mauritiana, D. yakuba, D. santomea</em> and <em>D. virilis</em>
AID  - 10.1101/096644
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 096644
4099  - http://biorxiv.org/content/early/2016/12/23/096644.short
4100  - http://biorxiv.org/content/early/2016/12/23/096644.full
AB  - Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. Studies in these species have been limited, however, by a paucity of genetic and transgenic reagents. Here we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein gene expressed in the eyes and a phiC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 86 transposable elements in D. virilis that will be useful for genetic mapping studies.