RT Journal Article
SR Electronic
T1 D-Alanine esterification of teichoic acids contributes to <em>Lactobacillus plantarum</em> mediated intestinal peptidase expression and <em>Drosophila</em> growth promotion upon chronic undernutrition
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 098434
DO 10.1101/098434
A1 Renata C. Matos
A1 Hugo Gervais
A1 Pauline Joncour
A1 Martin Schwarzer
A1 Benjamin Gillet
A1 Maria Elena Martino
A1 Pascal Courtin
A1 Sandrine Hughes
A1 Marie-Pierre Chapot-Chartier
A1 François Leulier
YR 2017
UL http://biorxiv.org/content/early/2017/01/05/098434.abstract
AB The microbial environment influence animal physiology. However, the underlying molecular mechanisms of such functional interactions are largely undefined. Previously, we showed that upon chronic undernutrition, strains of Lactobacillus plantarum, a dominant commensal partner of Drosophila, promote host juvenile growth and maturation partly via enhanced expression of intestinal peptidases. By screening a transposon insertion library of Lactobacillus plantarum in gnotobiotic Drosophila larvae, we identify a bacterial cell wall modifying machinery encoded by the pbpX2-dltXABCD operon that is critical to enhance host digestive capabilities and promote growth and maturation. Deletion of this operon leads to bacterial cell wall alteration with a complete loss of teichoic acids D-alanylation. We thus conclude that teichoic acids modifications participate in commensal-host interactions and specifically, D-alanine esterification of teichoic acids contributes to optimal L. plantarum mediated intestinal peptidase expression and Drosophila juvenile growth upon chronic undernutrition.Highlights- LpNC8 mutant library screening identifies genes affecting Drosophila growth promotion.- pbpX2-dlt operon is required for D-alanylation of teichoic acids and Drosophila growth.- Deleting the pbpX2-dlt operon alters host intestinal peptidase expression.- Peptidoglycan and pbpX2-dlt dependent signals are required for LpNC8 mediated growth promotion.eTOC blurb Animals establish interactions with their microbial communities that shape many aspects of their physiology including juvenile growth. However, the underlying molecular mechanisms are largely undefined. Matos et al. reveal that bacterial teichoic acids modifications contribute to host juvenile growth promotion.