PT  - JOURNAL ARTICLE
AU  - Natalie A. Petek
AU  - Alan I. Derman
AU  - Jason A. Royal
AU  - Joe Pogliano
AU  - R. Dyche Mullins
TI  - Polymer dynamics of Alp7A reveals two ‘critical’ concentrations that govern dynamically unstable actin-like proteins
AID  - 10.1101/098954
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 098954
4099  - http://biorxiv.org/content/early/2017/01/10/098954.short
4100  - http://biorxiv.org/content/early/2017/01/10/098954.full
AB  - Dynamically unstable polymers capture and move cellular cargos in both bacteria and eukaryotes, but the regulation of their assembly remains poorly understood. Here we describe polymerization of Alp7A, a bacterial Actin-Like Protein (ALP) that segregates the low copy-number plasmid pLS20 in Bacillus subtilis. Purified Alp7A forms dynamically unstable polymers with two critical points: an intrinsic critical concentration (0.6 μM), observed when ATP hydrolysis is blocked, and a dynamic critical concentration (10.3 μM), observed when ATP hydrolysis occurs. From biochemical and kinetic analysis, the intrinsic critical concentration reflects a balance between filament elongation and shortening, while the dynamic critical concentration reflects a balance between filament nucleation and catastrophic disassembly. Although Alp7A does not form stable polymers at physiological concentrations, rapid nucleation by an accessory factor, Alp7R, decreases the dynamic critical concentration into the physiological range. Intrinsic and dynamic critical concentrations are fundamental parameters that can be used to describe the behavior of all dynamically unstable polymers.