RT Journal Article
SR Electronic
T1 Systematic analysis of the effects of the DNA damage response network in telomere defective budding yeast
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 101253
DO 10.1101/101253
A1 Eva-Maria Holstein
A1 Greg Ngo
A1 Conor Lawless
A1 Peter Banks
A1 Matthew Greetham
A1 Darren Wilkinson
A1 David Lydall
YR 2017
UL http://biorxiv.org/content/early/2017/01/18/101253.abstract
AB Functional telomeres are critically important to eukaryotic genetic stability. Budding yeast is a powerful model organism for genetic analysis and yeast telomeres are maintained by very similar mechanisms to human telomeres. Scores of proteins and pathways are known to affect telomere function. Here, we report a series of related genome-wide genetic interaction screens performed on budding yeast cells with acute or chronic telomere defects. We examined genetic interactions in cells defective in Cdc13 and Stn1, affecting two components of CST, a single stranded DNA (ssDNA) binding complex that binds telomeric DNA. We investigated genetic interactions in cells with defects in Rfa3, affecting the major ssDNA binding protein, RPA, which has overlapping functions with CST at telomeres. We also examined genetic interactions in cells lacking EXO1 or RAD9, affecting different aspects of the DNA damage response in a cdc13-1 background. Comparing fitness profiles across the data sets allows us build up a picture of the specific responses to different types of dysfunctional telomeres. Our results show that there is no universal response to telomere defects. To help others engage with the large volumes of data we make the data available via two interactive web-based tools: Profilyzer and DIXY. Among numerous genetic interactions we found the chk1Δ mutation improved fitness of cdc13-1 exo1Δ cells more than other checkpoint mutations (ddc1Δ, rad9Δ, rad17Δ, rad24Δ), whereas in cdc13-1 cells the effects of all checkpoint mutations were similar. We find that Chk1 stimulates resection at defective telomeres, revealing a new role for Chk1 in the eukaryotic DNA damage response network.