RT Journal Article SR Electronic T1 Rapid de novo assembly of the European eel genome from nanopore sequencing reads JF bioRxiv FD Cold Spring Harbor Laboratory SP 101907 DO 10.1101/101907 A1 Hans J. Jansen A1 Michael Liem A1 Susanne A. Jong-Raadsen A1 Sylvie Dufour A1 Finn-Arne Weltzien A1 William Swinkels A1 Alex Koelewijn A1 Arjan P. Palstra A1 Bernd Pelster A1 Herman P. Spaink A1 Guido E. van den Thillart A1 Ron P. Dirks A1 Christiaan V. Henkel YR 2017 UL http://biorxiv.org/content/early/2017/01/20/101907.abstract AB We have sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large eukaryotic genomes. For this 860 Mbp genome, the entire computational process takes two days on a single CPU. The resulting genome assembly significantly improves on a previous draft based on short reads only, both in terms of contiguity (N50 1.2 Mbp) and structural quality. This combination of affordable nanopore sequencing and light-weight assembly promises to make high-quality genomic resources accessible for many non-model plants and animals.