PT  - JOURNAL ARTICLE
AU  - Kinshuk Banerjee
AU  - Anatoly B. Kolomeisky
AU  - Oleg A. Igoshin
TI  - Elucidating interplay of speed and accuracy in biological error correction
AID  - 10.1101/102608
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 102608
4099  - http://biorxiv.org/content/early/2017/01/23/102608.short
4100  - http://biorxiv.org/content/early/2017/01/23/102608.full
AB  - One of the most fascinating features of biological systems is the ability to sustain high accuracy of all major cellular processes despite the stochastic nature of underlying chemical processes. It is widely believed that such low errors are the result of the error correcting mechanism known as kinetic proofreading. However, it is usually argued that enhancing the accuracy should result in slowing down the process leading to so-called speed-accuracy trade-off. We developed a discrete-state stochastic framework that allowed us to investigate the mechanisms of the proofreading using the method of first-passage processes. With this framework, we simultaneously analyzed speed and accuracy of the two fundamental biological processes, DNA replication and tRNA selection during the translation. The results indicate that speed-accuracy trade-off is not always observed. However, when the trade-off is present, the biological systems tend to optimize the speed rather than the accuracy of the processes, as long as the error level is tolerable. Additional constraints due to the energetic cost of proofreading also play a role in the error correcting process. Our theoretical findings provide a new microscopic picture of how complex biological processes are able to function so fast with a high accuracy.