@article {Knoepp102756, author = {Fenja Knoepp and Zoe Ashley and Daniel Barth and Marina Kazantseva and Pawel P. Szczesniak and Wolfgang G. Clauss and Mike Althaus and Diego Alvarez de la Rosa and Martin Fronius}, title = {Mechanical activation of epithelial Na+ channel relies on an interdependent activity of the extracellular matrix and extracellular N-glycans of αENaC}, elocation-id = {102756}, year = {2017}, doi = {10.1101/102756}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Mechanotransduction describes how cells perceive their mechanical environment and mechanosensitive ion channels are important for this process. ENaC (epithelial Na+ channel)/DEG (degenerin) proteins form mechanosensitive ion channels and it is hypothesized their interaction with the extracellular matrix (ECM) via {\textquoteleft}tethers{\textquoteright} is required for mechanotransduction. Channels formed by vertebrate α, β and γ ENaC proteins are activated by shear force (SF) and mediate electrolyte/fluid-homeostasis and blood pressure regulation. Here, we report an interdependent activity of ENaC and the ECM that mediates SF effects in murine arteries and heterologously expressed channels. Furthermore, replacement of conserved extracellular N-glycosylated asparagines of αENaC decreased the SF response indicating that the attached N-glycans provide a connection to the ECM. Insertion of N-glycosylation sites into a channel subunit, innately lacking these motifs, increased its SF response. These experiments confirm an interdependent channel/ECM activity of mechanosensitive ENaC channel and highlight the role of channel N-glycans as new constituents for the translation of mechanical force into cellular signals.}, URL = {https://www.biorxiv.org/content/early/2017/01/24/102756}, eprint = {https://www.biorxiv.org/content/early/2017/01/24/102756.full.pdf}, journal = {bioRxiv} }