PT  - JOURNAL ARTICLE
AU  - Val F. Lanza
AU  - Fernando Baquero
AU  - José Luós Martónez
AU  - Ricardo Ramos-Ruóz
AU  - Bruno González-Zorn
AU  - Antoine Andremont
AU  - Antonio Sánchez-Valenzuela
AU  - Dusko Ehrlich
AU  - Sean Kennedy
AU  - Etienne Ruppe
AU  - Willem van Schaik
AU  - Rob J. Willems
AU  - Fernando de la Cruz
AU  - Teresa M. Coque
TI  - In-Depth Resistome Analysis by Targeted Metagenomics
AID  - 10.1101/104224
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 104224
4099  - http://biorxiv.org/content/early/2017/01/30/104224.short
4100  - http://biorxiv.org/content/early/2017/01/30/104224.full
AB  - We developed ResCap, a targeted sequence capture platform based on SeqCapEZ technology, to analyse resistomes and other genes related to antimicrobial resistance (heavy metals, biocides and plasmids). ResCap includes probes for 8,667 canonical resistance genes (7,963 antibiotic resistance genes and 704 genes conferring resistance to metals or biocides), plus 2,517 relaxase genes (plasmid markers). Besides, it includes 78.600 genes homologous to the previous ones (47,806 for antibiotics and 30,794 for biocide or metals). ResCap enriched 279-fold the targeted sequences detected by metagenomic shotgun sequencing and improves their identification. Novel bioinformatic approaches allow quantifying “gene abundance” and “gene diversity”. ResCap, the first targeted sequence capture specifically developed to analyse resistomes, enhances the sensitivity and specificity of available metagenomic methods to analyse antibiotic resistance in complex populations, enables the analysis of other genes related to antimicrobial resistance and opens the possibility to accurately study other complex microbial systems.