RT Journal Article SR Electronic T1 A toolkit for tissue-specific protein degradation in C. elegans JF bioRxiv FD Cold Spring Harbor Laboratory SP 104398 DO 10.1101/104398 A1 Shaohe Wang A1 Ngang Heok Tang A1 Pablo Lara-Gonzalez A1 Bram Prevo A1 Dhanya K. Cheerambathur A1 Andrew D. Chisholm A1 Arshad Desai A1 Karen Oegema YR 2017 UL http://biorxiv.org/content/early/2017/01/30/104398.abstract AB Proteins essential for embryo production, cell division, and early embryonic events are frequently re-utilized later in embryogenesis, during organismal development, or in the adult. Examining protein function across these different biological contexts requires tissue-specific perturbation. Here, we describe a method that utilizes expression of a fusion between a GFP-targeting nanobody and SOCS-box containing ubiquitin ligase adaptor to target GFP tagged proteins for degradation. When combined with endogenous locus GFP tagging by CRISPR-Cas9 or rescue of a null mutant with a GFP fusion, this approach enables routine and efficient tissue-specific protein ablation. We show that this approach works in multiple tissues—the epidermis, intestine, body wall muscle, sensory neurons, and touch neurons—where it recapitulates expected loss-of-function mutant phenotypes. The transgene toolkit and the strain set described here will complement existing approaches to enable routine analysis of the tissue-specific roles of C. elegans proteins.