PT  - JOURNAL ARTICLE
AU  - Christian Mayer
AU  - Christoph Hafemeister
AU  - Rachel C. Bandler
AU  - Robert Machold
AU  - Gord Fishell
AU  - Rahul Satija
TI  - Inhibitory neuron diversity originates from cardinal classes shared across germinal zones
AID  - 10.1101/105312
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 105312
4099  - http://biorxiv.org/content/early/2017/02/02/105312.short
4100  - http://biorxiv.org/content/early/2017/02/02/105312.full
AB  - During embryogenesis, the ventral telencephalon gives rise to a remarkable variety of GABAergic cell types, from long-range projection neurons to locally-projecting interneurons. Our current understanding of how this diversity is generated is far from complete. Using Drop-seq to profile the single-cell transcriptomes of over 36,000 ventral telencephalic cells isolated from three distinct germinal zones (the ganglionic eminences), we found that regardless of their origin and ultimate fate, all lineages transit through one of three cardinal transcriptional programs that define the earliest branch points in cell fate determination. Thus, the ability of these germinal zones to generate distinct cells likely relies on the small number of genes that are differentially expressed within each of these regions.