PT  - JOURNAL ARTICLE
AU  - Jesse Clarke
AU  - Noah Dephoure
AU  - Ira Horecka
AU  - Steven Gygi
AU  - Douglas Kellogg
TI  - A conserved signaling network monitors delivery of sphingolipids to the plasma membrane in budding yeast
AID  - 10.1101/105841
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 105841
4099  - http://biorxiv.org/content/early/2017/02/03/105841.short
4100  - http://biorxiv.org/content/early/2017/02/03/105841.full
AB  - In budding yeast, cell cycle progression and ribosome biogenesis are dependent upon plasma membrane growth, which ensures that events of cell growth are coordinated with each other and with the cell cycle. However, the signals that link the cell cycle and ribosome biogenesis to membrane growth are poorly understood. Here, we used proteome-wide mass spectrometry to systematically discover signals associated with membrane growth. The results suggest that membrane trafficking events required for membrane growth are required for normal sphingolipid-dependent signaling. A conserved signaling network plays an essential role in signaling by responding to delivery of sphingolipids to the plasma membrane. In addition, sphingolipid-dependent signals control protein kinase C (Pkc1), which plays an essential role in the pathways that link the cell cycle and ribosome biogenesis to membrane growth. Together, these discoveries provide new clues to how growth-dependent signaling controls cell growth and the cell cycle.