RT Journal Article
SR Electronic
T1 Synthetic DNA templates for the production of <em>in situ</em> hybridization probes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 108613
DO 10.1101/108613
A1 B. Rodney Jarvis
A1 Brian G. Condie
YR 2017
UL http://biorxiv.org/content/early/2017/02/14/108613.abstract
AB Generating RNA riboprobes for in situ hybridization generally requires the use of plasmids, which must be grown in bacteria, isolated, purified, and linearized prior to in vitro transcription. Here we report a simple method for generating DNA templates for the in vitro transcription of RNA probes from synthetic DNA (IDT gBlocks). Each synthetic DNA template contains sequences corresponding to the target mRNA flanked by bacteriophage promoters. Amplification of the template by a single round of PCR and subsequent in vitro transcription results in production of high quality RNA probes for in situ hybridization.